The movement biology or locomotory pattern in fish is employed as a reliable marker to assess native environmental (aquatic) condition. Disturbances in the ecosystem, even at the microenvironment level, can have a substantial impact on the biological system of the organism residing there, leading to notable variations in the behavioural manifestations that are mostly controlled by the underlying neural systems. Zebrafish (Danio rerio) are extremely sensitive to even the smallest environmental perturbation as evidenced by a noticeable shift in their locomotory pattern. This sensitivity is used to apply the theory in cognitive neuroscience research, which is based on the examination of collective movement decision-making.